An artificial Trachea (Windpipe) made from the patient stem cells

The trachea, or windpipe, is the tube that connects the nose and mouth to the lungs. It is an important part of the respiratory system because, when a person breathes in, air flows into the lungs through the windpipe. Any damage to it is potentially life-threatening because of its role in respiration.

Scientists in London created an artificial windpipe which was then coated in stem cells from the patient.

Crucially, the technique does not need a donor, and there is no risk of the organ being rejected. The surgeons stress a windpipe can also be made within days.

The 36-year-old cancer patient is doing well a month after the operation. Professor Paolo Macchiarini from Italy led the pioneering surgery, which took place at the Karolinska University Hospital. In an interview with the BBC, he said he now hopes to use the technique to treat a nine-month-old child in Korea who was born with a malformed windpipe or trachea.

Professor Macchiarini already has 10 other windpipe transplants under his belt – most notably the world’s first tissue-engineered tracheal transplant in 2008 on 30-year-old Spanish woman Claudia Costillo – but all required a donor.

The key to the latest technique is modelling a structure or scaffold that is an exact replica of the patient’s own windpipe, removing the need for a donor organ. To do this he enlisted the help of UK experts who were given 3D scans of the 36-year-old African patient, Andemariam Teklesenbet Beyene. The geology student currently lives in Iceland where he is studying for a PhD.

Using these images, the scientists at University College London were able to craft a perfect copy of Mr Beyene’s trachea and two main bronchi out of glass. This was then flown to Sweden and soaked in a solution of stem cells taken from the patient’s bone marrow. After two days, the millions of holes in the porous windpipe had been seeded with the patient’s own tissue.

Dr Alex Seifalian and his team used this fragile structure to create a replacement for the patient, whose own windpipe was ravaged by an inoperable tumour. Despite aggressive chemotherapy and radiotherapy, the cancer had grown to the size of a golf ball and was blocking his breathing. Without a transplant he would have died.

During a 12-hour operation Professor Macchiarini removed all of the tumour and the diseased windpipe and replaced it with the tailor-made replica. The bone marrow cells and lining cells taken from his nose, which were also implanted during the operation, were able to divide and grow, turning the inert windpipe scaffold into an organ indistinguishable from a normal healthy one.

And, importantly, Mr Beyene’s body will accept it as its own, meaning he will not need to take the strong anti-rejection drugs that other transplant patients have to. Professor Macchiarini said this was the real breakthrough.

In an other interview – see video – with the President of Harvard Bioscience, who also plays a key role in the regeneration of the trachea we can learn more about the progress of stem cells in regenerative medicine.

“Thanks to nanotechnology, this new branch of regenerative medicine, we are now able to produce a custom-made windpipe within two days or one week.” This is a synthetic windpipe. The beauty of this is you can have it immediately. There is no delay. This technique does not rely on a human donation.” He said many other organs could be repaired or replaced in the same way.

A month on from his operation, Mr Beyene is still looking weak, but well. Sitting up in his hospital bed, he said: “I was very scared, very scared about the operation. But it was live or die.” He says he is looking forward to getting back to Iceland to finish his studies and then returning to his home in Eritrea where he will be reunited with his wife and young family, and meet his new three-month-old child.

He says he is eternally grateful to the medical team that has saved his life.


Related Stories:
Synthetic transplant surgery 07 JULY 2011, HEALTH
Windpipe transplant breakthrough 19 NOVEMBER 2008, HEALTH
Stem cell windpipe op ‘success’ 19 MARCH 2010, HEALTH

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